Maturation of dendritic cells from ovarian cancer patients
Zavadova E., Savary C. A.1, Templin S., Verschraegen C.
F.2, Freedman R. S.
Department of Gynecologic Oncology,1Department of
Surgical Oncology, 2Gynecologic Medical Therapeutics, The University
of Texas M. D. Anderson Cancer Center.
Cancer Chemother Pharmacol (2001) 48: 289-296.
Purpose: Dendritic cells (DC) are the most potent
antigen-presenting cells of the immune system. We have shown that DC, defined as
LN-DR+ leukocytes from the ascites of patients with
ovarian or peritoneal carcinoma, have the cell surface characteristics of
immature cells. Moreover, p70 interleukin-12 has not been detected in the
ascites of ovarian cancer patients in vivo. In the current study, we examined
the effects of in vitro treatment of DC from peripheral blood and ascites
samples of patients with ovarian cancer with either cytokines or proteolytic
enzymes (polyenzyme preparation).
leukocytes from the ascites of 15 patients or peripheral blood from 9 patients
with epithelial ovarian cancer were cultured with tissue culture medium
containing either papain, trypsin and chymotrypsin for 5-7 days or recombinant
human granulocyte-macrophage colony-stimulating factor, tumor necrosis factor
a and interleukin-4 (complete medium) or tissue culture
Results: Phenotypic analysis of DC
obtained on days 5-7 of the culture showed higher proportions of
CD83+, CD40+ and CD80+ cells when cultured with
cytokines or enzymes as compared with DC cultured with medium alone. Stimulation
of allogeneic T cells was detected by the mixed leukocyte reaction (MLR) and
higher concentrations of IL-12 were detected after growing these cells in the
presence of cytokines or enzymes as compared to tissue culture medium
Conclusion: Our studies demonstrate for the first
time that DC obtained from the peritoneal cavity and peripheral blood of ovarian
cancer patients after culturing in the presence of a polyenzyme preparation,
will undergo maturation. Further studies are warranted to determine whether
polyenzyme preparations facilitate DC maturation in vivo.
Keywords Dendritic cells • Ovarian cancer • Polyenzyme