Systémová enzymoterapie pro odborníky

Stimulation of reactive oxygen species production and cytotoxicity in human neutrophils in vitro and after oral administration of a polyenzyme preparation

Eva Zavadova, Lucia Desser and Thomas Mohr

Institute of Applied and Experimental Oncology, University of Vienna, Austria

Cancer Biotherapy 1995: Vol.10, No. 2, pp. 147-152
10. Tagung über Infektionen in der gynäkologie und der Gebursthilfe, München, März 1995, publ. in Eur. J. Immunol. Dis. 1996: Suppl. Vol. 1, No. 1, pp. 42 - 44

WE 56 (2-04-2)

Abstract

Polymorphonuclear neutrophils (PMN) can be primed for enhanced release of reactive oxygen species (ROS) by exposure to cytokines and biological response modifiers. ROS are considered to possess tumoricidal activity.
The polyenzyme preparation Wobenzym^R (WE) contains pancreatin, papain, bromelain, trypsin and chymotrypsin and is used in adjuvant tumor therapy. We investigated killing of WE-exposed PMN against tumor cells and analyzed WE influence on ROS production in a chemiluminescence assay in PMN in vitro and in vivo.
Depending on dose WE stimulates the cytotoxic capacity of PMN in vitro against tumor cells (50 mg/ml: p<0.01).
Exposure of PMN to Wobenzym caused a time-dependent significant (p<0.02) increase in release of ROS. Similarly, oral administration of Wobenzym to healthy volunteers (n = 28) resulted in significant increases (p<0.01) in ROS production, depending on dose (peak with 20 tablets) and time (peak 4 hours after Wobenzym administration).
In contrast, ROS production was not elevated in the PMN of healthy volunteers receiving placebo (n=8) or no treatment (n=16).
These findings point to an immunomodulatory capacity of WE in adjuvant tumor therapy.


Úvod pro odborníky O firmě Kontakty Pomáháme druhým Poradna
Zánět a jeho ovlivnění SET Zvýšení využitelnosti antibiotik proteolytickými enzymy Angiologie, flebologie Diabetologie Gynekologie Chirurgie, traumatologie, ortopedie Imunologie Lymfologie ORL Pediatrie Revmatologie Sportovní medicína Stomatologie Urologie Literatura Časopisy s Impact faktorem Ostatní literatura SPC Wobenzym SPC Phlogenzym	Stimulation of reactive oxygen species production and cytotoxicity in human neutrophils in vitro and after oral administration of a polyenzyme preparation Eva Zavadova, Lucia Desser and Thomas Mohr Institute of Applied and Experimental Oncology, University of Vienna, Austria Cancer Biotherapy 1995: Vol.10, No. 2, pp. 147-152 10. Tagung über Infektionen in der gynäkologie und der Gebursthilfe, München, März 1995, publ. in Eur. J. Immunol. Dis. 1996: Suppl. Vol. 1, No. 1, pp. 42 - 44 WE 56 (2-04-2) Abstract Polymorphonuclear neutrophils (PMN) can be primed for enhanced release of reactive oxygen species (ROS) by exposure to cytokines and biological response modifiers. ROS are considered to possess tumoricidal activity. The polyenzyme preparation Wobenzym^R (WE) contains pancreatin, papain, bromelain, trypsin and chymotrypsin and is used in adjuvant tumor therapy. We investigated killing of WE-exposed PMN against tumor cells and analyzed WE influence on ROS production in a chemiluminescence assay in PMN in vitro and in vivo. Depending on dose WE stimulates the cytotoxic capacity of PMN in vitro against tumor cells (50 mg/ml: p<0.01). Exposure of PMN to Wobenzym caused a time-dependent significant (p<0.02) increase in release of ROS. Similarly, oral administration of Wobenzym to healthy volunteers (n = 28) resulted in significant increases (p<0.01) in ROS production, depending on dose (peak with 20 tablets) and time (peak 4 hours after Wobenzym administration). In contrast, ROS production was not elevated in the PMN of healthy volunteers receiving placebo (n=8) or no treatment (n=16). These findings point to an immunomodulatory capacity of WE in adjuvant tumor therapy.
	Lék pro vnitřní užití. Čtěte pečlivě příbalovou informaci. Inzertní stránky společnosti MUCOS Pharma CZ, s.r.o. - tel: (+420)-267 750 003